By Wayne W Umbreit
Meant for researchers in utilized microbiology and environmental engineers, this ebook covers such subject matters as environmental review of biotechnological procedures and microbial alterations of haloaromatic and haloaliphatic compounds.
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A more complex arrangement described by Stull and Herreid (1949) included a mercury diffusion pump and certainly required the services of an expert glass blower to assemble it. On the basis of the data presented the performance of their apparatus did not seem to be significantly superior to that of other simpler designs. The apparatus described by Campbell and Pressman (1944) requires the services of a professional glass blower but it has the advantage of not requiring a separate insulated container for the refrigerant.
Viruses, on the other hand, survive as well, or better, when frozen rapidly. Greiff (1960) showed that survival of influenza virus after slow cooling was approximately equivalent to survival after rapid cooling and others have indicated that infectivity of viruses was best maintained by rapid freezing. A rationalization of why slow freezing is superior to fast freezing for the preservation of bacteria and other cells is that slow freezing allows time for the cells to adapt to the new environment produced by freezing of the preparation.
Short sections of %-in. tubing are fitted into the tees to facilitate attaching the %-in. gum rubber tubing. As illustrated, the main condenser was a 500-ml. Pyrex filter flask. The %-in. tubing was extended into the flask so that most of the water vapor would be directed down and condensed near the bottom of the flask. If it extends too far below the level of the refrigerant and is cooled below OOC. the %-in. tube may become plugged before drying is completed. The secondary trap, which effectively prevented contamination of the pump oil with water, consisted of a loop of tygon tubing submerged in the same ethanol-dry-ice bath as the flask.
Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit